MINFLUX 3D – Precision in Fluorescence Microscopy
MINFLUX (minimal fluorescence flux) is the fastest method for localizing fluorescent molecules. With its revolutionary MINFLUX nasoscope, Abberior Instruments, is raising the bar for molecular nan oscale tracking with a world-record temporal resolution of 100 µs, opening new doors for life scientists across all disciplines. MINFLUX 3D sets a new gold standard in molecular imaging, combining unprecedented spatial resolution with world-record imaging speed. Developed by abberior, this revolutionary fluorescence microscope offers 1–3 nanometer precision in 3D, allowing scientists to resolve structures at the scale of single molecules—far beyond the capabilities of traditional superresolution methods.
Features
- Unmatched Resolution: Achieves localization precision of 1–3 nm in 3D — 10× better than other superresolution techniques and 100× better than confocal imaging.
- Ultra-Fast Tracking: Monitors molecular motion at up to 10,000 localizations per second (10 kHz), enabling dynamic studies with 100 µs time resolution.
- Photon Efficiency: Requires fewer emitted photons, reducing bleaching and allowing for longer observation times of single molecules.
- True 3D Imaging: Localizes fluorescent molecules precisely along x, y, and z axes in biological samples.
- Large Field of View: Enables high-resolution imaging across fields of view as large as 10 x 15 μm.
- Low Drift Sensitivity: Inherently less susceptible to drift and molecular orientation artifacts compared to camera-based systems.
- iMSPECTOR Software: Intuitive control of nanometer-resolution imaging and millisecond-scale tracking at the push of a button.
- Innovative Localization Principle: Uses a central intensity-zero excitation beam to achieve maximal spatial accuracy with minimal emission.
Applications
- Live-Cell Imaging: Capture real-time biological processes with nanometer precision and millisecond resolution.
- Structural Biology: Resolve molecular structures at the scale of individual proteins and complexes.
- Neuroscience: Track synaptic proteins and molecular interactions in neural cells.
- Microbiology: Observe subcellular components and processes in bacteria and other microorganisms.
- Drug Discovery: Visualize drug-target interactions at the molecular level in living cells.
- Molecular Biophysics: Analyze the dynamics of molecular machines and intracellular transport mechanisms.
- Cell Signaling Studies: Follow signaling cascades and receptor dynamics in 3D at unprecedented speed.
Specifications
| Parameter | Specification |
|---|---|
| Resolution | 1–3 nm in 3D |
| Field of View | Large, with dimensions of 10 × 15 μm |
| Tracking Speed | Up to 10 kHz, resolving molecular motion every 100 μs |
| Methodology | Combines principles of coordinate-stochastic localization and coordinate-targeted super-resolution (like STED) |
| Implementation | Can be integrated with standard microscope platforms |
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