PhyPrep System – Automated Plasmid Purification
The Biotage® PhyPrep System is the only fully automated plasmid purification solution designed for maxiprep, megaprep, and gigaprep scales. It eliminates labor-intensive manual steps, reducing processing time while ensuring high-quality, endotoxin-free plasmid DNA. With intuitive software and pre-aliquoted reagents, the PhyPrep delivers reproducible results that free skilled staff from repetitive workflows. Biotage®PhyPrep is designed to make automation simple and easy to introduce to your laboratory. Cell pellet preparation makes use of pre-aliquoted buffers so that you can get your samples prepared within minutes in a reproducible manner without the need to dispense and measure solutions.
Feauters
- Performance by Scale – Operates across maxi, mega, and giga plasmid purification with yields of ≥1/5/10 mg DNA.
- High Purity Output – Endotoxin-free plasmid DNA with <0.1 EU/μg, A260/A280 > 1.8, A260/A230 > 2.0.
- Time-Saving Automation – Fully automated runs with throughput of up to 4 samples per run.
- Intuitive Software – 12" touchscreen interface and simple scale selection for easy operation.
- Consistent Sample Preparation – Pre-aliquoted reagents minimize pipetting errors and improve reproducibility.
- Certified System – CE, FCC, CB, and NRTL certified for compliance in regulated environments.
Applications
- Transient transfection experiments requiring endotoxin-free plasmid DNA.
- Cloning workflows in molecular biology and biotechnology.
- Gene therapy research and plasmid-based therapeutic development.
- Biopharmaceutical production, ensuring high-quality DNA for downstream applications.
- Academic and industrial labs looking to reduce manual plasmid prep workload and variability.
Specifications
| Parameter | Specification |
|---|---|
| Weight | 23 kg (50 lbs), excluding computer |
| Dimensions (W x D x H) | 530 x 720 x 830 mm (20.9" x 28.4" x 32.7") |
| Interfaces | 12" touchscreen, Ethernet port |
| Power Consumption | 820 VA (100 VA for instrument only) |
| Purification Scale | Maxiprep, Megaprep, Gigaprep |
| Plasmid Yield | ≥1 mg (maxi), ≥5 mg (mega), ≥10 mg (giga) |
| Throughput | 1–4 samples per run |
| Processing Time | Maxiprep: 42–70 min; Megaprep: 76–142 min; Gigaprep: 151–298 min |
| Elution Volume | 5 ml (maxi), 18 ml (mega), 28 ml (giga) |
| Endotoxin Levels | <0.1 EU/µg plasmid DNA |
| Purity Ratios | A260/A280 > 1.8; A260/A230 > 2.0 |
| Certifications | CE, FCC, CB, NRTL |
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