STEDYCON 2

STEDYCON 2 is a compact, plug-and-play fluorescence imaging upgrade that transforms any existing microscope into a powerful confocal, STED super-resolution, and TIMEBOW lifetime imaging system. Designed in a sleek, modern housing that matches the MIRAVA platform, STEDYCON 2 integrates all lasers, detectors, scanners, and autofocus modules into one maintenance-free device. With super-resolution down to 30 nm, fully integrated FLIM powered by PicoQuant, and browser-based intuitive software, STEDYCON 2 delivers high-end imaging performance at a significantly lower cost than conventional confocal systems. Installation takes only minutes — simply connect to a camera port, select your fluorophores, and start imaging. Perfect for labs seeking cutting-edge imaging capabilities without replacing their microscope frame, STEDYCON 2 offers exceptional reliability, alignment-free operation, and advanced imaging workflows for modern research.

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Features
  • Confocal, STED, and FLIM lifetime imaging in one compact module
  • Super-resolution STED imaging with up to 30 nm resolution
  • Integrated TIMEBOW lifetime imaging (FLIM) powered by PicoQuant
  • Pulsed 775 nm STED laser + excitation lasers at 488, 561, 640 nm
  • Optional additional 405 nm CW excitation line
  • QUAD scanner with high-speed, high-precision imaging
  • 4 high-sensitivity APD detectors for maximum signal efficiency
  • STEADYFOCUS: continuous hardware autofocus, no additional optics required
  • All lasers aligned by design — maintenance-free optical path
  • Intuitive, browser-based STEDYCON smart control software
  • 1-click STED activation, fast workflow setup
  • Multi-positioning, tiling, 3D imaging, time-lapse, and Z-stacks
  • Export to LiGHTBOX for advanced post-processing
  • Low cost of ownership compared to traditional confocal systems
  • Compatible with nearly any microscope frame (upright or inverted)
Applications
  • Super-resolution fluorescence microscopy
  • Live-cell imaging and dynamic cellular studies
  • FLIM lifetime imaging for biochemical and metabolic analysis
  • Multicolor 2D and 3D imaging
  • Neuroscience and synaptic imaging
  • Cell biology and structural biology
  • Microbial imaging
  • Tissue and organoid imaging
  • Quantitative imaging and biophysics
  • Academic research labs and imaging facilities
  • Upgrading older microscopes to modern high-performance imaging
Specifications
Category Specification
Imaging Modes 2D/3D confocal, STED super-resolution, FLIM (TIMEBOW)
STED Resolution <40 nm, typically 30 nm
STED Laser Pulsed 775 nm
Excitation Lasers 488 nm, 561 nm, 640 nm; optional 405 nm
Scanner QUAD scanner
Detectors 4 × APD detectors
Pinhole Motorized
Autofocus STEADYFOCUS continuous hardware autofocus
Software Browser-based STEDYCON smart control
Software Features 1-click STED, TIMEBOW FLIM, tiling, 3D, time-lapse, multi-position, navigation, LiGHTBOX export
Microscope Compatibility Any frame with camera port (upright/inverted)
Optical Alignment All lasers aligned by design; maintenance-free
Installation Time A few minutes